Equipment

Macromolecular Crystallography Branch

  • Rigaku MicroMax 007HF X-ray generator with VariMax HighFlux optic and R-AXIS IV image plate detector
  • Oxford liquid nitrogen crystal cryo-cooling system with auto refill
  • Art Robbins Instruments GRYPHON liquid-handling crystallization robot JANSi UV and Olympus microscopes for crystal viewing and documentation
  • 3 low-vibration incubators (4-20oC) for storage of crystallization experiments
  • Six terabyte RAID5 file system mounted on a Dell PowerEdge R310 server for crystallographic computing and data storage
  • Dell workstation, installed with crystallographic software suites, is available for in-house and remote diffraction data processing and structure determination
  • HKL2000 and D* software for data processing and scaling
  • CCP4, PHENIX, CNS, COOT, and PYMOL software packages for crystal structure determination and visualization
  • ALTAS software package for small-angle X-ray scattering data analysis
  • Crystallization kits, including Hampton, JCSG, Wizard Suites

Bacterial Protein Expression [ISB 203, 207, 209]

  • 3 New Brunswick Innova 4330 refrigerated floor shaker for bacterial growth
  • New Brunswick Bioflo/Celligen 115 fermentor with Eppendorf 7L vessel and Thermoflex 1400 recirculating water bath
  • Sorvall RC6+ Floor centrifuge with Fiberlite F10 4x10000 LEX and Fiberlite F21-8x50Y rotors
  • Bio-Rad semi-dry trans-blot cells for western blot for evaluating protein expression
  • JASCO V630 UV/Visible Spectrophotometer

Insect Cell Protein Expression [Skaggs 057]

  • SterilGard III Advance Class II Biological Safety Cabinet Hood for carry out tissue culture work
  • 2 Innova 4430 incubator shakers for maintenance and scale-up insect cells growth
  • Thermo Forma Direct Hear CO2 Incubation with HEPA filter for bacmid transfection
  • Nikon Eclipse Ti-SM inverted microscope
  • Lift Technology Countess II cell counter
  • Binder Drying Oven for baking sterilized culture flask

Protein Purification [ISB 207, Skaggs 057]

  • 2 Amersham Pharmacia AKTA-FPLC and 1 GE AKTA-Pure fast performance Chromatography System for high-through protein purification
  • Avestin Emulsiflex C5 High Pressure Homogenizer for cell lysis
  • Beckman L8-70M Ultracentrifuge
  • Large collection of commercial pre-packed and in-house packed affinity, ion-exchange, and hydrophobic chromatographic columns
  • 6 GE Superdex 75 10/300 and GE Superdex 200 10/300GL size exclusion columns
  • Wyatt TREOS II multi-angle light scattering

Protein characterization [ISB 207, Skaggs 057]

  • Microcal VP-ITC Calorimeter for determine binding constant of protein-protein or protein-ligand interactions
  • Molecular Devices Spectramax M2E spectrophotometer for UV/Visible/fluorescence analysis of enzymatic kinetic
  • Applied Photophysics SX20 Stopped-flow spectrometer for studying fast reaction mechanisms in solution
  • Perkin Elmer LS55 luminescence spectrometer
  • Jasco J-815 Circular Dichroism Spectrometer
  • Bruker MOLTI-TOF Mass Spectroscopy [NMR/Mass Spectroscopy Core]
  • Biacore X100

Small Molecule Capabilities

  • Bruker D8 Venture fixed Chi dual source small molecule diffractometer with both Mo Kα radiation (TRIUMPH monochromator) and Cu microfocus (IμS) Kα radiation with PHOTON 100 CMOS detector
  • Oxford cryostream 700 Plus liquid nitrogen cryo-cooling system with auto refill
  • Meiji optical microscope with various crystal manipulation tools
  • Two Dell workstation running Windows 7, Olex2.0 and the APEX 2 software suite including ShelXTL
  • Access to the Cambridge Crystal Structural Database
  • Powder X-ray diffraction capabilities (optimal for small quantities of sample)
Bruker D8 Venture Bruker D8 Venture