Illumina MiSeq

The Genomics Core offers in house next generation sequencing services on the MiSeq™ Personal Sequencing System™ from Illumina. We are able to generate up to 15 Gb of data per run with up to 50 M high quality, 2 X 300 Paired-End Reads. The Genomics Core Facility also offers Illumina’s full line of sample preparation, library construction, in addition to Custom NGS tools.

Sequencing

  • Paired End Reads (PR): Libraries are sequenced from both ends.
  • Single Read (SR): Libraries
  • Index: Individual libraries

Price List

Request Sequncing Services

Illumina Information

Genomics Customers submitting prepared libraries for sequencing MUST make sure libraries contain the necessary P5 and P7 flowcell sequence, Read 1, Index, Read 2 primer sites, and proper sequence diversity specified for the MiSeq™  platform.  Please consult with the genomics core prior to constructing custom libraries to make sure that your library designs are compatible with the MiSeq™  system.

Libraries MUST be submitted in no less than 10 nM final concentration.  For multiplexed libraries submit your pool at 10 nM.  Individual library submissions that require pooling will incur extra QC, quantification, and pooling fees.

Quality check prior to sample submission should be done via fluorometry quantification and BioAnalyzer/ TapeStation analysis.  The Genomics Core will perform these quality checks at the request of the investigator for an additional cost.  qPCR of final library is performed on all prepared NGS submissions and is included in the cost of sequencing. 

Any Illumina MiSeq kits are available (http://www.illumina.com/systems/miseq/kits.html )

PhiX control is added to each run.

Mailed sample submission: Make sure to email Tamara Max to let her know samples have been shipped the day they are shipped.

Next Generation Sequencing Data Retrieval

Data will be automatically generated on the Illumina BaseSpace website.

You will need to create an Illumina account to access your data. Once your run is complete, you will be able to download your sequence data and view run metrics on BaseSpace.

Illumina supported commercial Kits

Custom Illumina Library Preparation

Mailed sample submission: Make sure to email Tamara Max to let her know samples have been shipped the day they are shipped.