Research Core Facilities

University of Montana Genomics Core Facility

University of Montana Genomics Core Facility is a service-based core laboratory providing genetic and genomic resources to researchers at universities, institutions, and government laboratories on the state and national levels. The newly expanded core includes technical support in molecular biology applications including Sanger DNA sequencing, fragment analysis, Illumina library preparation, Illumina sequencing, and other more specialized techniques on a case-by-case basis. Additional equipment includes Covaris E220 ultrasonicator, epMotion 5075TMX liquid handler, Agilent TapeStation, BioTek HT plate reader and qPCR Thermocycler. The facility is housed in the Division of Biological Sciences. Our mission is to provide rapid, high quality services at very low cost to the investigators.

Lab location: ISB 007, ISB 323

Contact
David Xing, Lab Manager

Office: ISB 321

Phone: (406) 243-5210
Denghui.xing@umontana.edu

Fluorescence Cytometry Core

The mission of the Fluorescence Cytometry Core is to provide state of the art research instrumentation utilizing fluorescence labeling of cells and tissues as well as scientific expertise to UM researchers to aid them in their efforts to unravel disease processes to ultimately benefit human health.

Equipment
BD FACSAria™: 3 laser, 10 color analyzer and high speed sorter
BD FACSCalibur™: 1 laser, 3 color analyzer
CompuCyte iCys Laser Scanning Cytometer™: 4 laser, 4 color image cytometer
Luminex™: 2 laser, multi-analyte analyzer
Miltenyi Biotec autoMacs™: magnetic bead sorter
Zeiss™: Fluorescence Microscope with digital camera

Location
Center for Environmental Health Sciences
The University of Montana
Skaggs Building 052
Missoula, MT 59812

Contact
Phone: (406) 243-4974

Pam Shaw, Core Facilitator
pamela.shaw@umontana.edu 

Inhalation and Pulmonary Physiology Core

The Inhalation and Pulmonary Physiology Core was established in 2010 with the mission of providing state-of-the-art technology for inhalation toxicology. Resources and expertise are provided to investigators, laboratory personnel, and students in the use of inhalation exposure chambers, murine pulmonary function measurements, and air sample collection and analysis. Core resources are available to researchers within and outside the CEHS whose emphasis is on understanding the etiology and progression of acute and chronic disorders resulting from inhalation exposures, developing and testing methods for monitoring external and internal (i.e., biomarkers) exposures in humans, and developing new preventive and therapeutic approaches for airborne gases- and particle-induced disease.

Location
Center for Environmental Health Sciences
The University of Montana
Skaggs Building 061
Missoula, MT 59812

Contact
Phone: (406) 243-4579

Mary Buford, Core Manager
mary.buford@umontana.edu

Molecular Histology and Fluorescence Imaging

The mission of the Molecular Histology & Fluorescence Imaging Core Facility is to assist scientists at the University of Montana and across the state of Montana with projects involving the identification, visualization, and localization of proteins in samples from cell culture and sectioned tissues as well as live cell imaging.  The facility has two components: histology and imaging.  The purpose of the Molecular Histology side of the core is to provide researchers with the resources to prepare tissue sections for histological and immunohistochemical staining.  The Fluorescence Imaging side of the core provides researchers with analyzable images of cells/tissues. The core contains both the equipment and the technical assistance for acquiring and analyzing images taken from both the light and confocal microscope in both fixed tissue and live cells. The newly acquired Olympus FV1000 Scanning Confocal-TIRF Imaging Workstation (S10RR024535) has significantly enhanced the research and educational capacity at the University of Montana and surrounding area.

Location
Center for Environmental Health Sciences
Molecular Histology and Fluorescent Imaging Core
Skaggs Building  070 , 052A, 060C
University of Montana Missoula, MT 59812

Contact
Phone: (406) 243-2225

Lou Herritt, Staff Scientist
lou.herritt@umontana.edu

Mass Spectrometry Core

The Mass Spectrometry Core Facility provides centrally managed and maintained facilities essential for a broad array of research in the chemical and biomedical sciences at UM.  It contains three Mass Spectrometers: an Agilent GC/MS, a Bruker microflex MALDI-ToF Mass Spectrometer, and a Bruker-Daltonics ion trap Mass Spectrometer interfaced with a micro-capillary LC. These instruments provide the tools needed to characterize the structural and dynamic properties of biological macromolecules and the components of complex protein mixtures (proteomics), biopolymer sequence analysis, and small molecule characterization within environmental chemistry applications.

Location
Center for Biomolecular Structure and Dynamics
Interdisciplinary Sciences Building 106
Missoula, MT 59812

Contact
Phone: (406) 243-6003

Dr. Earle R. Adams, Mass Spectrometry Core Director
earle.adams@umontana.edu cbsd@umontana.edu

EMTrix

EMtrix is a remotely accessible electron microscopy facility. The fully staffed facility supports diverse research, teaching and outreach activities that require the use of transmission or scanning electron microscopy (TEM, SEM). Principal equipment includes a Hitachi 7100 TEM and a Hitachi S-4700 cold field emission SEM. Both microscopes can be accessed remotely via the internet. The fully equipped specimen preparatory laboratory includes ultramicrotomes, knifemakers, sputter coater, critical point dryer, vacuum evaporators, embedding ovens and light microscopes.

Location

Contact

Jim Driver

jim.driver@mso.umt.edu

Other Resources

The University of Montana has a comprehensive list of university-wide resources, including facilities and equipment.