Christopher Comer, Dean
BIOB 425--Advanced Cellular & Molecular Biology
BIOB 565--Membrane Dynamics Research Seminar
BIOB/BCH 547--Experimental Molecular, Cellular & Chemical BIology
Ph.D. University of Wisconsin-Madison, 1994
Postdoctoral Training, Stanford University, 1994-1998
Nearly every aspect of cell function depends upon the delivery of cellular products to their proper intracellular locations. Membrane-bound organelles receive their proteins, lipids and other constituents through inter-organelle exchanges mediated by ~50 nanometer spherical transport vesicles that bud from the donor compartment and are properly targeted to, and fuse with, the acceptor compartment. Transport vesicles contain special membrane proteins, called SNAREs, that are hypothesized to allow vesicles to dock and fuse at the appropriate acceptor membrane. Other proteins such as vesicle coats, rabs, SM proteins and membrane tethers work with and regulate SNAREs to ensure accurate vesicle biogenesis, delivery and fusion. It is hard to imagine proteins more central to the organization and differentiation of intracellular membranes. Furthermore, many diseases result from defects in trafficking processes.
Dr. Hay's laboratory is trying to understand the molecular mechanism of action, regulation, and protein interactions of these vesicle trafficking proteins, using mammalian endoplasmic reticulum (ER)-to-Golgi trafficking as a model system. A wide variety of techniques are employed in this laboratory, including in vitro reconstitutions of transport processes in permeabilized cells, microscopy, and in vitro biochemistry.
PLEASE VISIT THE HAY LAB WEBSITE FOR MORE INFORMATION http://www.cas.umt.edu/dbs/labs/hay/
Field of Study
Molecular Cell Biology
Dalu Xu, Ashwini Joglekar, Antionette L. Williams, and Jesse C. Hay. 2000. Subunit Structure of a Mammalian ER/Golgi SNARE Complex. Journal of Biological Chemistry 275:39631-39639.
Dalu Xu and Jesse C. Hay. 2004. In Vitro Reconstitution of Homotypic COPII Vesicle Fusion to Generate a Pre-Golgi Intermediate Compartment. Journal of Cell biology 167:997-1003.
Marvin Bentley, Yingjian Liang, Karl Mullen, Dalu Xu, Elizabeth Sztul, and Jesse C. Hay. 2006. SNARE Status Regulates Tether Recruitment and Function in Homotypic COPII Vesicle Fusion. Journal of Biological Chemistry 281:38825-38833.
Huaqing Cai, Sidney Yu, Shekar Menon, Yiying Cai, Darina Lazarova, Chunmei Fu, Karin Reinisch, Jesse C. Hay, and Susan Ferro-Novick. 2007. TRAPPI Tethers COPII Vesicles by Binding the Coat Subunit Sec23. Nature 445:941-944.
Jesse C. Hay. 2007. Calcium: a Fundamental Regulator of Membrane Fusion? EMBO Reports 8:236-240.
Marvin Bentley, Deborah Nycz, Ashwini Joglekar, and Jesse C. Hay. 2010. Vesicular Calcium Regulates Coat Retention, Fusogenicity and Size of Pre-Golgi Intermediates. Molecular Biology of the Cell 21:1033-1046.
Nandhakumar Thayanidhi, Jared Helm, Deborah C. Nycz, Marvin Bentley, Yingjian Liang and Jesse C. Hay. 2010. Alpha-Synuclein Delays Endoplasmic Reticulum (ER)-to-Golgi Transport in Mammalian Cells by Antagonizing ER/Golgi SNAREs. Molecular Biology of the Cell 21:1850-1863.
Christopher Lord, Deepali Bhandari, Shekar Menon, Majid Ghassemian, Deborah Nycz, Jesse Hay, Pradipta Ghosh, and Susan Ferro-Novick. 2011. Sequential interactions with Sec23 control the direction of vesicle traffic. Nature 473:181-186.
Nandhakumar Thayanidhi, Yingjian Liang, Haruki Hasegawa, Deborah Nycz, Viola Oorschot, Judith Klumperman, and Jesse C. Hay. 2012. R-SNARE YKT6 Resides in Membrane-Associated Protease-Resistant Protein Particles and Modulates Cell Cycle Progression When Over-Expressed. Biology of the Cell 104:397-417.
Meg Trahey, Hyung Suk Oh, Craig E. Cameron, and Jesse C. Hay. 2012. Poliovirus Infection Transiently Increases COPII Vesicle Budding. Journal of Virology 86:9675-9682.
Robert Grabski, Jesse Hay, and Elizabeth Sztul. 2012. Tethering Factor P115: a New Model for Tether-SNARE Interactions. Bioarchitecture 2:1-6.
Deepali Bhandari, Jinzhong Zhang, Shekar Menon, Christopher Lord, Shuliang Chen, Jared R Helm, Kevin Thorsen, Kevin D Corbett, Jesse C Hay, Susan Ferro-Novick. 2013. Sit4p/PP6 regulates ER-to-Golgi traffic by controlling the dephosphorylation of COPII coat subunits. Molecular Biology of the Cell 24:2727-38.
J. Helm, M. Bentley, K. Thorsen, T. Wang, L. Foltz, J. Klumperman, J. C. Hay. 2014. Apoptosis-linked Gene-2/Sec31 Interactions Regulate ER-to-Golgi Transport: a Potential Effector Pathway for Luminal Calcium. Journal of Biological Chemistry 289:23609-28.
Ting Wang, Robert Grabski, Elizabeth Sztul and Jesse C. Hay. 2015. P115-SNARE interactions: a dynamic cycle of P115 binding monomeric SNARE motifs and releasing assembled bundles. Traffic 16:148–171.
Ting Wang and Jesse C. Hay. 2015. Alpha-synuclein Toxicity in the Early Secretory Pathway: How It Drives Neurodegeneration in Parkinsons Disease. Frontiers Neuroscience 9:433.
Mariah Rayl, Mishana Truitt, Aaron Held, John Sargeant, Kevin Thorsen, and Jesse C. Hay. 2016. Peflin is a Negative Regulator of ER-to-Golgi Transport. PLoS one, 11(6):e0157227.
Molecular Cell Biology
Specialized Research Interests
Intracellular membrane trafficking and secretion.
BIOH 483W - Neuroscience Research Lab
BIOB 425 - Adv. Cell & Molecular Biology
Neuroscience Research Interests
Vesicle transport in the secretory pathway and its relationship to neurodegeneration.