Genomics Core Services

UMGC Core Logo

University of Montana Genomics Core offers a variety of services from fragment analysis or Sanger sequencing using capillary electrophoresis to NGS library preparation and sequencing on Illumina MiSeq. Training of faculty, staff, or students is available on any core instrument, library preparation technique, or general molecular biology techniques.

NGS

The University of Montana Genomics Core Laboratory houses an Illumina MiSeq benchtop sequencer.  The MiSeq instrument utilizes a single flowcell and reagent kit in each run. There are a variety of kits and configurations to support any level of sequencing need.  The MiSeq can be used for paired-end as well as single end sequencing.  MiSeq reagent kits v2 (50, 300, and 500 cycle kits) expect sequence from 10-15 million reads (24 to 30 million reads for paired end) and v3 kits (150 and 600 cycle kits) will yield 22-25 million reads (44 to 50 million reads for paired end).

The University of Montana Genomics Core Laboratory contains all the necessary instrumentation for library preparation.

As a service we prepare Illumina libraries from purchased kits as well as custom libraries including genomic DNA-seq and RNA-seq methods.>

The length of Nanopore sequencing read is limited only by the length of input DNA and RNA molecules. For those who seek long read length, UMGC provide timely MinION services. 

Provide your high molecular weight DNA samples that meet the following criteria, you will get long-read sequencing data around 8 Gb: A260/280 ≈1.8, A260/230 = 2.0-2.2; More than 2.5 µg of DNA (≥ 50 ng/µL) with average fragment length ≥ 30Kb (Note: Starting with lower amounts of input material, and/or impure samples, can affect library preparation efficiency, leading to reduced sequencing throughput). For DNA samples with low molecular weight, amplicon or cDNA, please contact UMGC for details.

For direct RNA sequencing, please provide more than 500ng of Poly(A) RNA or Contact UMGC for non-poly(A) RNA.

UMGC is equipped with Molecular and Genetics expertise. UMGC provides services for original research projects (ORPs) defined as those that require customized library constructions including Single Cell Whole Genome Sequencing (SCG-Seq), Chromatin-immuno Precipitation followed by sequencing (ChIP-Seq), RNA-immuno Precipitation followed by sequencing (RIP-Seq), Assay of Transposase-Accessible Chromatin sequencing (ATAC-Seq), Demethylase-thermostable group II intron RT tRNA sequencing (DM-tRNA-seq), to just name a few.The responsibilities and rights of the UMGC and its collaborators involved in ORPs are outlined as the followings.  
 
ORPs will start with well-defined scientific goal(s). UMGC will work closely with collaborators to reach an agreement on the research approaches to take, the estimated timelines for the project, the data to obtain, and the major contents in the final project report. UMGC will frequently communicate, formally (with written reports) and informally (orally, or short emails), with collaborators on all ORPs. Depending on the data collected, UMGC and collaborators will re-evaluate the ORP at each stage and decide if the project will continue as planned, needs to be re-approached, or stopped altogether.
 
The collaborator will provide funds for all required consumables and equipment usage (if not available in UMGC or the laboratory of the collaborator), and compensate for the labor and intellectual input of the UMGC. The compensation for UMGC may be an hourly fee ($46/hour) or calculated at a predetermined cost in grant applications.
 
Should ORP-relevant data be used for publication, the UMGC is entitled to co-authorship on the publication. Should ORP-relevant data or ideas be used for grant applications, UMGC asks to be listed as funding collaborator in the grant proposal. UMGC is happy to assist the writing of the grant proposals for the relevant portions.

ABI 3130 Genetic Analyzer

The University of Montana Genomics Core Laboratory provides full service cycle sequening of purified DNA template, PCR product, or plasmid.  This includes dye terminator removal, and data generation on the ABI 3130xl genetic analyzer. Sequencing can be done using either standard sequencing primers provided by the core or user provided custom primers. Technical support and troubleshooting is also provided at customers request. Sequence service and sample submission information.

The University of Montana Genomics Core Laboratory is equipped with an Applied Biosystems Genetic Analyzer for use in Fragment Analysis including GeneScan size standards in AFLP, SNP, and MSAT analysis. Fragment analysis service and sample submission information.