Denghui David Xing, Ph.D.
Genomics Laboratory Manager
ISB 321(office) & 007 (Lab)
Genomics Core Services
University of Montana Genomics Core offers a variety of services from fragment analysis or Sanger sequencing using capillary electrophoresis to NGS library preparation and sequencing on Illumina MiSeq. Training of faculty, staff, or students is available on any core instrument, library preparation technique, or general molecular biology techniques.
Illumina Sequencing, MiSeq
The University of Montana Genomics Core Laboratory houses an Illumina MiSeq benchtop sequencer. The MiSeq instrument utilizes a single flowcell and reagent kit in each run. There are a variety of kits and configurations to support any level of sequencing need. The MiSeq can be used for paired-end as well as single end sequencing. MiSeq reagent kits v2 (50, 300, and 500 cycle kits) expect sequence from 10-15 million reads (24 to 30 million reads for paired end) and v3 kits (150 and 600 cycle kits) will yield 22-25 million reads (44 to 50 million reads for paired end).
Illumina Library Preparation
The University of Montana Genomics Core Laboratory contains all the necessary instrumentation for library preparation.
As a service we prepare Illumina libraries from purchased kits as well as custom libraries including genomic DNA-seq and RNA-seq methods.>
Nanopore Sequencing, MinION
The length of Nanopore sequencing read is limited only by the length of input DNA and RNA molecules. For those who seek long read length, UMGC provide timely MinION services.
Provide your high molecular weight DNA samples that meet the following criteria, you will get long-read sequencing data around 8 Gb: A260/280 ≈1.8, A260/230 = 2.0-2.2; More than 1 µg of DNA (≥ 20 ng/µL) with average fragment length ≥ 30Kb (Note: Starting with lower amounts of input material, and/or impure samples, can affect library preparation efficiency, leading to reduced sequencing throughput). For DNA samples with low molecular weight, amplicon or cDNA, please contact UMGC for details.
For direct RNA sequencing, please provide more than 500ng of Poly(A) RNA or Contact UMGC for non-poly(A) RNA.
Research Project Services
ABI 3130 Genetic Analyzer
The University of Montana Genomics Core Laboratory provides full service cycle sequening of purified DNA template, PCR product, or plasmid. This includes dye terminator removal, and data generation on the ABI 3130xl genetic analyzer. Sequencing can be done using either standard sequencing primers provided by the core or user provided custom primers. Technical support and troubleshooting is also provided at customers request. Sequence service and sample submission information.
The University of Montana Genomics Core Laboratory is equipped with an Applied Biosystems Genetic Analyzer for use in Fragment Analysis including GeneScan size standards in AFLP, SNP, and MSAT analysis. Fragment analysis service and sample submission information.